Federally Regulated Testing
DOT Urine Specimen Collection
Fully compliant collections for safety-sensitive employees under 49 CFR Part 40. We handle the process so you stay audit-ready.
What Is DOT Drug Testing?
The U.S. Department of Transportation (DOT) requires drug and alcohol testing for employees in safety-sensitive positions across regulated industries including trucking, aviation, rail, transit, pipeline, and maritime.
All DOT testing must follow strict federal procedures outlined in 49 CFR Part 40, which governs everything from specimen collection and chain of custody to laboratory analysis and Medical Review Officer (MRO) review.
DOT Agencies We Serve
We collect specimens for employees regulated by all six DOT operating agencies.
Federal Motor Carrier Safety Administration
Commercial truck and bus drivers
Federal Aviation Administration
Aviation safety-sensitive personnel
Federal Railroad Administration
Railroad employees in covered positions
Federal Transit Administration
Mass transit safety-sensitive workers
Pipeline & Hazardous Materials Safety Administration
Pipeline operations personnel
United States Coast Guard
Maritime industry workers
The Collection Process
Every collection follows the exact federal protocol required by 49 CFR Part 40.
Donor Identification
We verify the donor's identity using a government-issued photo ID before any collection begins.
Federal CCF Completion
A five-part Federal Custody and Control Form (CCF) is completed accurately, documenting the collection event from start to finish.
Specimen Collection
The donor provides a urine specimen under controlled conditions that prevent adulteration or substitution, per Part 40 protocols.
Specimen Integrity Check
Temperature is measured immediately. The specimen is checked for validity indicators before sealing.
Split Specimen Sealing
The specimen is split into Bottle A and Bottle B, sealed with tamper-evident tape, and the donor initials the seals.
Laboratory Shipment
Specimens are shipped to a SAMHSA-certified laboratory via secure chain of custody for analysis.
MRO Review
A licensed Medical Review Officer reviews all non-negative results, contacts the donor if needed, and issues a final verified result to the employer.
Required Testing Circumstances
DOT regulations require testing under six specific circumstances.
Pre-Employment
Required before a safety-sensitive employee begins performing covered functions.
Random Testing
Unannounced, scientifically random selection from a pool of safety-sensitive employees throughout the year.
Post-Accident
Required following certain accidents or incidents involving safety-sensitive employees.
Reasonable Suspicion
Ordered by a trained supervisor who observes specific, articulable signs of drug or alcohol use.
Return-to-Duty
Required before an employee who violated drug/alcohol policy returns to safety-sensitive functions.
Follow-Up Testing
Unannounced testing following return-to-duty, as directed by a Substance Abuse Professional (SAP).
DOT 5-Panel Drug Test
All DOT urine tests screen for the following five drug categories as mandated by federal regulation:
Marijuana (THC)
Including CBD-derived THC
Cocaine
Cocaine and metabolites
Opioids
Codeine, morphine, heroin, oxycodone, oxymorphone, hydrocodone, hydromorphone
Amphetamines
Amphetamine, methamphetamine, MDMA, MDA
Phencyclidine (PCP)
PCP and metabolites
Ready to Schedule a DOT Collection?
We make compliance straightforward. Contact us to schedule a collection or ask about setting up an ongoing testing program.